Longevity Testing Guide: 15 Biomarkers for Healthy Aging

Longevity testing shifts the focus from treating illness to proactively managing health as you age. These tests examine your body’s internal aging processes to help you improve your healthspan—the quality of your years, not just the quantity.

Analyzing biomarkers linked to aging pathways lets you detect early signs of metabolic dysfunction, oxidative stress, or cellular senescence. This knowledge empowers you to address potential issues before they become serious, putting you in control.

This guide details 15 essential biomarkers for healthy aging. Learn their roles, why they matter for longevity, and how your results can inform targeted lifestyle choices for lasting health.

What is Longevity Testing?

A longevity test is a blood test that analyzes key biomarkers to predict how your body is aging. The primary goal is health,span optimization, which means maximizing the years you live in good health with full functional capacity.

This proactive approach differs from traditional tests that mainly focus on diagnosing illnesses that are already present.

Modern lab tests typically include: 

• Markers of oxidative stress (e.g., 8 OHdG)

• Indicators of metabolic health (e.g., HbA1c, lipid profiles)

• Signs of inflammation, known as inflammatory mediators (e.g., hs CRP, IL 6)

• Biomarkers for cellular aging (e.g., Klotho, senescence associated β galactosidase)

Many of these markers are combined in advanced longevity blood panels like Jinfiniti’s AgingSOS®. Our test evaluates 22 biomarkers tied to mitochondrial health, inflammation, and gene regulation.

By closely examining these biomarkers, you can catch potential issues early and take targeted action to support long-term health and slow biological aging.

15 Key Longevity Blood Test Biomarkers

Here are 15 key biomarkers in a longevity blood test that may help predict health outcomes and promote overall wellness.

1. Albumin

Albumin is a protein made by the liver that helps regulate blood volume and keeps fluids in your bloodstream. It also transports hormones and binds to toxins, helping your body stay balanced and functioning properly.

When albumin levels are low, it can signal issues like chronic inflammation, malnutrition, or liver problems.¹ These low levels often lead to increased frailty and slower recovery, especially as we age.

A study in BMC Geriatrics found that many centenarians maintain healthy albumin levels well into their later years.² This suggests strong liver function and the body’s ability to keep up with protein production and repair.

2. ALP (Alkaline Phosphatase) & ALT (Alanine Aminotransferase)

ALP (Alkaline Phosphatase) and ALT (Alanine Aminotransferase) are key enzymes that give clues about liver and bone health.

ALP helps break down proteins and supports bone growth and repair, while ALT is mainly found in the liver and rises when liver cells are damaged.

Elevated ALP can point to bone disorders or bile duct problems, while high ALT levels often signal liver inflammation or stress.³ Tracking both markers helps spot potential issues before they become serious.

ALT usually drops with age, but that doesn’t always mean better liver health.⁴ Centenarians with stable ALT levels likely have good liver detox functions, which may be key to their long, healthy lives.

3. Creatinine (CRE)

Creatinine (CRE) is a waste product filtered out by the kidneys, and its levels reflect how well your kidneys are functioning.⁵

Lower creatinine levels usually indicate that the kidneys filter waste efficiently and that muscle mass is well-preserved.

As we get older, creatinine levels usually go up because kidney function naturally declines. But studies on centenarians in Springer Link showed that they often keep their creatinine levels low, suggesting their kidneys remain strong.⁶

Moreover, reduced creatinine levels in older adults can signal a slower kidney decline. This is a key indicator of healthy aging and longevity.

4. Creatine Kinase (CK)

Elevated CK (Creatine Kinase) levels can indicate muscle damage or metabolic stress from intense activity, injury, or inflammation.⁷

While it’s not always directly linked to aging, CK’s role in muscle health is crucial. Maintaining muscle mass and strength is key to staying mobile and independent as we age.

Optimal muscle function reduces fall risk, improves balance, and maintains physical vitality, making CK monitoring valuable for health and longevity.⁸

5. Reactive Oxygen Metabolite (ROM)

Reactive Oxygen Metabolite (ROM) levels measure the burden of oxidative stress on the body. High ROM levels can accelerate DNA damage and contribute to cellular aging.⁹

Consistently elevated ROM is a sign of the body’s struggle to manage oxidative stress. Luckily, antioxidants like vitamin E help reduce ROM levels, protecting against oxidative damage.¹⁰

Keeping ROM levels in check may help slow aging and reduce the risk of chronic diseases. This is why antioxidants are crucial for healthy aging and longevity.

6. Total Antioxidant Capacity (TAC)

Total Antioxidant Capacity (TAC) measures how well your body fights oxidative stress.¹¹ Low TAC levels suggest weaker defenses, which may increase your risk of chronic diseases.

TAC shows the balance between oxidative stress and your body’s repair systems. An imbalance toward too much stress speeds up aging and increases risk of disease.

High TAC levels are essential for healthy aging. They indicate your body effectively protects itself from free radical damage, supporting long term health and longevity.

7. DNA Damage (8-OHdG)

DNA Damage (8-OHdG) is a biomarker that indicates oxidative damage to your DNA.¹² Higher levels, especially in older individuals, suggest that the body’s repair mechanisms are slowing down.

When DNA repair isn’t functioning properly, it can lead to accelerated aging and increased disease risk. The good news is that nutritional interventions like zinc and vitamin E can help lower 8-OHdG levels.¹³

8. Intracellular NAD+

NAD+ is crucial for mitochondrial function and activating sirtuins, which help regulate energy metabolism and support cellular health. Unfortunately, as we age, NAD+ levels naturally decline.¹⁴

This decline in NAD+ is linked to various age-related health issues, including fatigue and metabolic dysfunction. Research has explored supplementation with NAD+ precursors as a potential way to reverse these effects.¹⁵

Jinfiniti’s Intracellular NAD+ Test is designed to accurately test your cellular NAD+ levels and provide actionable insights for restoring them.

9. Vitamin D

Vitamin D helps regulate immune function and supports bone health. When you have low levels, it increases your risk for chronic diseases and shortens your healthspan.¹⁶

Maintaining optimal vitamin D levels supports the immune system and reduces the risk of frailty, especially as we age.¹⁷ It helps your body fight infections and keeps your bones strong, which is essential for overall health.

10. Glycated Serum Protein (GSP)

Glycated Serum Protein (GSP) shows your short term blood sugar management. Used alongside HbA1c, it helps assess your diabetes risk.¹⁸ GSP specifically reflects glucose levels over a more recent period.

Maintaining stable blood sugar levels, or glycemic control, is essential for health. It protects against vascular and neurological damage.¹⁹ 

Monitoring both GSP and HbA1c provides a clearer view of your potential for diabetes. This allows you to take steps to safeguard your health.

11. Blood Lipids (HDL, LDL, Triglycerides)

Blood lipids such as HDL, LDL, and triglycerides play important roles in heart health. HDL helps protect the heart, while high levels of LDL and triglycerides signal greater risk for cardiovascular disease.

Research in The Lancet found that people who live past 100 typically have lower cholesterol levels, suggesting healthier lipid profiles support cardiovascular health.²⁰

Keeping track of your lipids can guide you toward a heart-healthy lifestyle that promotes a longer, healthier life.

12. Uric Acid

Uric acid forms when the body breaks down purines. Too much of it can lead to gout, kidney stones, or signal metabolic issues.²¹ 

On the flip side, uric acid also acts as an antioxidant in small amounts.²² It helps neutralize free radicals and may play a role in protecting cells from oxidative stress.

Centenarians often maintain lower yet balanced levels, suggesting they benefit from its protective effects without the downsides.²³

Keeping uric acid in check can help you avoid metabolic risks and support healthy aging.

13. Klotho

Klotho is an anti-aging protein that supports kidney function, mineral balance, and brain health while regulating key longevity processes.²⁴

As we get older, Klotho levels naturally decline, which may contribute to faster aging and greater disease risk. 

A study published in Metabolites showed that people with higher Klotho levels tend to age more slowly and stay healthier longer.²⁵

Monitoring Klotho gives you insight into your biological age beyond just life expectancy. Supporting healthy levels may help resist age-related decline.

14. Inflammation (hs-CRP, IL-1β, IL-6, IL-8, TNF-α)

Chronic inflammation strongly affects aging and disease risk. It quietly chips away at health over time, often without early symptoms.

Markers like hs-CRP, IL-6, IL-1β, IL-8, and TNF-α reveal how much immune stress your body is under.²⁶ When these stay elevated, they can damage tissues and fuel issues like heart disease, diabetes, and cognitive decline.

This long term, low intensity inflammation called “inflammaging” is a critical target for healthy aging.²⁷

Regular exercise, omega 3 consumption, and stress management practices can effectively keep it under control.

15. Senescence-Associated β-Galactosidase (SABG or β-gal)

Senescence-Associated β-Galactosidase (SABG or β-gal) is a marker that identifies senescent cells, which stop multiplying but stay alive in the body. These “zombie” cells build up over time and release harmful substances that cause inflammation and damage tissues.²⁸

Research shows that this accumulation leads to chronic disease as we age.²⁹ Senolytic treatments may work to remove these cells and improve tissue health.

Clinical Applications and Future Trends

Longevity testing is entering an exciting new phase due to advances in omics and AI biomarker discovery. These technologies help measure biological aging more accurately than just tracking calendar years.

Epigenetic clocks such as DNAmAge and PhenoAge are leading this trend.³⁰ They estimate your biological age and predict future health risks with greater precision than traditional methods.

Another important tool is GlycanAge.³¹ It tracks glycosylation patterns that influence immune function and overall health resilience, giving valuable insights into your potential lifespan.

These health assessments are becoming part of everyday life. Biomarkers help us track how our bodies respond to nutrition, exercise, and stress on a regular basis.

This provides actual data that can guide personalized health decisions rather than relying on guesswork.

Test Your Biomarkers with Jinfiniti’s AgingSOS Panel

Tracking your longevity biomarkers can transform how you age. These key health indicators help you make better choices and prevent potential health problems before they appear.

Jinfiniti’s AgingSOS panels make it easy to track these biomarkers. The starter panel gives you a solid foundation by measuring essential markers, while the advanced panel dives deeper with a complete selection of blood biomarkers. Armed with this data, you can take action today for optimal health and well-being tomorrow.

Start your journey to healthier aging today. Whether you’re just getting started or want a comprehensive analysis, Jinfiniti has a panel that fits your needs.

Referenced Sources:

1. https://www.ncbi.nlm.nih.gov/books/NBK459198/ ↩︎
2. https://pmc.ncbi.nlm.nih.gov/articles/PMC7325239/ ↩︎
3. https://www.ncbi.nlm.nih.gov/books/NBK459201/ ↩︎
4. https://onlinelibrary.wiley.com/doi/full/10.1002/jcla.24937 ↩︎
5. https://www.sciencedirect.com/topics/nursing-and-health-professions/creatinine-blood-level ↩︎
6. https://link.springer.com/article/10.1007/s11357-023-00936-w ↩︎
7. https://pmc.ncbi.nlm.nih.gov/articles/PMC3263635/ ↩︎
8. https://pmc.ncbi.nlm.nih.gov/articles/PMC7466089/ ↩︎
9. https://www.sciencedirect.com/science/article/pii/S2001037019304209 ↩︎
10. https://pmc.ncbi.nlm.nih.gov/articles/PMC7692274/ ↩︎
11. https://pmc.ncbi.nlm.nih.gov/articles/PMC11351343/ ↩︎
12. https://www.mdpi.com/2076-3921/12/2/517 ↩︎
13. https://pmc.ncbi.nlm.nih.gov/articles/PMC7692274/ ↩︎
14. https://pmc.ncbi.nlm.nih.gov/articles/PMC11552646/ ↩︎
15. https://iadns.onlinelibrary.wiley.com/doi/10.1002/fft2.511 ↩︎
16. https://pmc.ncbi.nlm.nih.gov/articles/PMC5440113/ ↩︎
17. https://onlinelibrary.wiley.com/doi/full/10.1002/jcsm.13525 ↩︎
18. https://www.sciencedirect.com/science/article/abs/pii/S1877117319300183 ↩︎
19. https://www.ncbi.nlm.nih.gov/books/NBK279093/ ↩︎
20. https://www.thelancet.com/journals/lanhl/article/PIIS2666-7568(23)00260-X/fulltext ↩︎
21. https://pmc.ncbi.nlm.nih.gov/articles/PMC3247913/ ↩︎
22. https://pmc.ncbi.nlm.nih.gov/articles/PMC4062324/ ↩︎
23. https://pmc.ncbi.nlm.nih.gov/articles/PMC4062324/ ↩︎
24. https://pmc.ncbi.nlm.nih.gov/articles/PMC4608225/ ↩︎
25. https://pmc.ncbi.nlm.nih.gov/articles/PMC10673288/ ↩︎
26. https://www.researchgate.net/publication/230567645_Elevated_serum_IL-6_IL-8_MCP-1_CRP_and_IFN-gamma_levels_in_10-_to_11-year-old_boys_with_increased_BMI ↩︎
27. https://pmc.ncbi.nlm.nih.gov/articles/PMC5850851/ ↩︎
28. https://pmc.ncbi.nlm.nih.gov/articles/PMC6949083/ ↩︎
29. https://pmc.ncbi.nlm.nih.gov/articles/PMC11203944/ ↩︎
30. https://pmc.ncbi.nlm.nih.gov/articles/PMC9373966/ ↩︎
31. https://pmc.ncbi.nlm.nih.gov/articles/PMC4049143/ ↩︎